Cow CAMP-dependent protein kinase type II-alpha regulatory subunit (PRKAR2A) ELISA Kit

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AE25991BO
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Assay Kits, ELISA, Cow ELISA, Abebio
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Product Name Cow CAMP-dependent protein kinase type II-alpha regulatory subunit (PRKAR2A) ELISA Kit
Species Bovine (Bos taurus, Cattle)
Description This assay has high sensitivity and excellent specificity for detection of Bovine PRKAR2A. No significant cross-reactivity or interference between Bovine PRKAR2A and analogues was observed.
Background The inactive holoenzyme of PKA is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of PKA have been identified in humans.
PRKAR2a is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of PKA. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
Abbreviation PRKAR2A
Synonyms MGC3606, PKR2, PRKAR2, OTTHUMP00000210266|cAMP-dependent protein kinase regulatory subunit RII alpha|cAMP-dependent protein kinase, regulatory subunit alpha 2|protein kinase A, RII-alpha subunit
Method Sandwich ELISA
Detection Range Request Information
Sensitivity Request Information
Sample Types Serum, Plasma, Other biological fluids.
Uniprot No. P00515
Principle of the Assay This assay employs a two-site sandwich ELISA to quantitate PRKAR2A in samples. An antibody specific for PRKAR2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKAR2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKAR2A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKAR2A bound in the initial step. The color development is stopped and the intensity of the color is measured.

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