Cow CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) ELISA Kit

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KTE10180
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Assay Kits, ELISA, Cow ELISA, Abbkine
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Product Name Cow CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) ELISA Kit
Species Cow
Description This Bovine CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKAR2B in samples. An antibody specific for PRKAR2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKAR2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKAR2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKAR2B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans.
PRKAR2b is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity.
Synonyms PRKAR2B, PRKAR2, RII-BETA, H_RG363E19.2, WUGSC:H_RG363E19.2, cAMP-dependent protein kinase type-beta regulatory chain, cAMP-dependent protein kinase, regulatory subunit beta 2
Method Sandwich ELISA
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene ID 282463
Uniprot Accession ID P31322
Intended Use For Research Use Only

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