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|Product Name||Cow Collagen alpha-1 (XVIII) chian (COL18A1) ELISA Kit|
|Species||Bovine (Bos taurus, Cattle)|
|Description||This assay has high sensitivity and excellent specificity for detection of Bovine COL18A1. No significant cross-reactivity or interference between Bovine COL18A1 and analogues was observed.|
|Background||Collagen, type XVIII, alpha 1, also known as COL18A1,alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains.The proteolytically produced C-terminal fragment of type XVIII collagen is endostatin, a potent antiangiogenic protein. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Two transcript variants encoding different isoforms have been found for this gene.|
|Synonyms||FLJ27325, FLJ34914, KNO, KNO1, MGC74745, alpha 1 type XVIII collagen|antiangiogenic agent|endostatin|multi-functional protein MFP|
|Detection Range||Request Information|
|Sample Types||Serum, Plasma, Other biological fluids.|
|Principle of the Assay||This assay employs a two-site sandwich ELISA to quantitate COL18A1 in samples. An antibody specific for COL18A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCOL18A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COL18A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COL18A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.|
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