Human HMGCR(3-Hydroxy-3-methylglutaryl CoA Reductase) ELISA KitÊ

Catalog
E-EL-H2472
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Assay Kits, ELISA, Human ELISA, Elabscience
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Product Name Human HMGCR(3-Hydroxy-3-methylglutaryl CoA Reductase) ELISA Kit
Species Human
Description This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HMGCR . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HMGCR and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HMGCR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HMGCR. The concentration of Human HMGCR in samples can be calculated by comparing the OD of the samples to the standard curve.
Synonyms 3-Hydroxy-3-methylglutaryl CoA Reductase
Method Sandwich ELISA
Detection Range 0.625-40ng/mL
Sensitivity 0.375ng/mL
Sample Types Serum, Plasma, Cell supernatant
Uniprot No. P04035
FDA Status For research use only, not for use in diagnostic procedures.
Antigen Name HMGCR

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