Human ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit

Catalog
E-EL-H0025
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Assay Kits, ELISA, Human ELISA, Elabscience
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Product Name Human ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit
Species Human
Description This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ECF/CCL11 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ECF/CCL11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ECF/CCL11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ECF/CCL11. The concentration of Human ECF/CCL11 in samples can be calculated by comparing the OD of the samples to the standard curve.
Synonyms Eosinophil Chemotactic Factor
Method Sandwich ELISA
Detection Range 15.625-1000pg/mL
Sensitivity 9.375pg/mL
Sample Types Serum, Plasma, Cell supernatant
Uniprot No. P51671
FDA Status For research use only, not for use in diagnostic procedures.
Antigen Name ECF/CCL11

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