Actin

Catalog
03-62201
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Proteins, Native Protein
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Product Name Actin
Description Actin purified from rabbit muscle
Background The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
Synonyms ACTA, ASMA, CFTD, MPFD, NEM1, NEM2, NEM3, CFTD1, CFTDM
Source Rabbit muscle
Molecular Weight 43000
Form Lyophilized, salt balanced.
Reconstitution When reconstituted with 100ul H20 dist., a final solution volume of 100 ul will contain 10mM Tris/HCI, pH 8.0, 0.2mM CaCl2, 0.2mM ATP, 1mM DTT, 0.5% (weight/volume) SDS with a protein concentration of 1mg/ml (according to protein dry weight).
Working Dilutions Optimal dilution should be determined by user
Purity > 98%, SDS-PAGE
Applications ELISA, Immunization, Protein standard in 1D and 2D SDS gelelectrophoresis
Isoelectric Point pI 5.4
Storage 4C (lyophilized); at -20C (solution, aliquot)
References Spudich, J.A. and Watt, S. (1971) J. Biol. Chem. 246: 4866

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