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|Product Name||Alpha Actinin|
|Description||Alpha Actinin purified from chicken gizzard|
|Background||Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.|
|Synonyms||F-actin cross-linking protein, actinin 1 smooth muscle, alpha-actinin cytoskeletal isoform, alpha-actinin-1, non-muscle alpha-actinin-1|
|Form||Lyophilized, salt balanced.|
|Reconstitution||When reconstituted with 250ul H20 dist, a final solution volume of 250 ul will contain 20mM Tris/Acetate, pH 7.6, 0.1mM EDTA, 2mM DTT, 20mM NaCl with a protein concentration of 1mg/ml (according to Bradford protein assay)|
|Working Dilutions||Optimal dilution should be determined by user|
|Purity||> 95%, SDS-PAGE|
|Applications||ELISA, Immunization, Protein standard in 1D and 2D SDS gelelectrophoresis|
|Isoelectric Point||pI 5.8|
|Storage||4C (lyophilized); at -20C (solution, aliquot)|
|References||Duhaiman, A.S. and Bamburg, J.R. (1984) Biochem. 23: 1600
Feramisco, J.R. and Burridge, K. (1980) J. Biol. Chem. 255: 1194
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