Alpha Actinin

Proteins, Native Protein
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Product Name Alpha Actinin
Description Alpha Actinin purified from chicken gizzard
Background Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.
Synonyms F-actin cross-linking protein, actinin 1 smooth muscle, alpha-actinin cytoskeletal isoform, alpha-actinin-1, non-muscle alpha-actinin-1
Source Chicken gizzard
Molecular Weight 95000
Form Lyophilized, salt balanced.
Reconstitution When reconstituted with 250ul H20 dist, a final solution volume of 250 ul will contain 20mM Tris/Acetate, pH 7.6, 0.1mM EDTA, 2mM DTT, 20mM NaCl with a protein concentration of 1mg/ml (according to Bradford protein assay)
Working Dilutions Optimal dilution should be determined by user
Purity > 95%, SDS-PAGE
Applications ELISA, Immunization, Protein standard in 1D and 2D SDS gelelectrophoresis
Isoelectric Point pI 5.8
Storage 4C (lyophilized); at -20C (solution, aliquot)
References Duhaiman, A.S. and Bamburg, J.R. (1984) Biochem. 23: 1600

Feramisco, J.R. and Burridge, K. (1980) J. Biol. Chem. 255: 1194

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