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|Description||The vector pST1374-N-NLS-Flag-Cas9 is is designed for expression of Cas9 nuclease for mammalian gene modification studies using CRISPR/Cas9 technology. Cas9 and the fluorescent protein are co-expressed from a bidirectional CMV promoter. The Cas9 nuclease is fused with Nuclear Localization Signal (NLS) and flag tag in the N terminus under the control of CMV promoter, nuclear localization signals (NLS) at the N-terminus is to ensure efficient import of the hspCas9 protein into the nucleus, and flag-tag is used for detection and purification of the recombinant Cas9 protein.|
|Package Contents||20 µl pST1374-N-NLS-Flag-Cas9-EGFP Vector (7.5 ng/µl)|
|Plasmid Identity||- Vector identity was confirmed by sequencing.
- A260/A280: 1.8-2.0
|Storage||Store at -20°C. Avoid repeated freeze/thaw cycles. Shipped with Ice Gel Packs.|
|Storage Buffer||10 mM Tris-HCl (pH 8.0), 1 mM EDTA (pH 8.0)|
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