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|Product Name||PYCRL, 1-274aa, Human|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MGSMAAAEPS PRRVGFVGAG RMAGAIAQGL IRAGKVEAQH ILASAPTDRN LCHFQALGCR TTHSNQEVLQ SCLLVIFATK PHVLPAVLAE VAPVVTTEHI LVSVAAGVSL STLEELLPPN TRVLRVLPNL PCVVQEGAIV MARGRHVGSS ETNLLQHLLE ACGRCEEVPE AYVDIHTGLS GSGVAFVCAF SEALAEGAVK MGMPSSLAHR IAAQTLLGTA KMLLHEGQHP AQLRSDVCTP GGTTIYGLHA LEQGGLRAAT MSAVEAATCR AKELSRK|
|Background||PYCRL belongs to the pyrroline-5-carboxylate reductase family and functions as a homodecamer. It may play a critical role in proline bio-synthesis. Proline functions as a non-enzymatic antioxidant to minimize damage caused by reactive oxygen species (ROS) in microorganisms, animals and plants. In the last step of proline biosynthesis, PYCRL catalyzes the reduction of aldehyde dehydrogenase 4A1 (ALDH4A1) to proline using NAD(P)H as the cofactor. Recombinant human PYCRL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Concentration||0.5 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol, 1mM DTT|
|Intended Use||For Research Use Only|
|Molecular Weight||19.0kDa (160aa), confirmed by MALDI-TOF|
|NCBI Accession #||AAH07993|
|Purity||> 90% by SDS-PAGE|
|References||Nocek B., et al. (2005) J Mol Biol. 354:91-106.
Krishnan N., et al. (2008) Free Radic Biol Med. 44: 671-681.
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||Pyrroline-5-carboxylate reductase 3, P5C reductase 3, P5CR 3, pyrroline-5-carboxylate reductase 3|
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