Rabbit Anti-brain tissue antibody (ABAb) ELISA Kit

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KTE90130
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Product Name Rabbit Anti-brain tissue antibody (ABAb) ELISA Kit
Description Rabbit Anti-brain tissue antibody (ABAb) ELISA Kit has high sensitivity and excellent specificity for detection of Rabbit ABAb. No significant cross-reactivity or interference between Rabbit ABAb and analogues was observed. Neuromodulating activity of anti-brain autoantibodies obtained from electroshocked (ECS) rats was tested on the neurons of isolated suboesophageal ganglion of the snail Helix pomatia. In 16 out of 18 spontaneously active (pacemaker) neurons. ECS IgG containing anti-brain autoantibodies induced short-lasting epileptiform discharges and membrane depolarization. Membrane input resistance and time constant decreased, while membrane capacitance increased after addition of ECS IgG. Amplitude of evoked action potential (AP) decreased, whereas AP duration, rise time and fall time slightly increased. Thus, anti-neural autoantibody-positive IgG from rats with experimental epilepsy, but not autoantibody-negative IgG from control rats, significantly affected the bioelectrical properties of the isolated snail neurons. These results suggest that anti-neural autoantibodies present in epileptic animals are capable of influencing in vivo the function of the brain neurons. This Rabbit Anti-brain tissue antibody (ABAb) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ABAb. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated ABAb and incubated. The competitive inhibition reaction is launched between with HRP labeled ABAb and unlabeled ABAb with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of ABAb in the sample. The color development is stopped and the intensity of the color is measured.
Synonyms ABAb
Method Sandwich ELISA
Detection Range Please inquire
Sensitivity Please inquire
Reactivity Rabbit
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Storage 2-8°C
Supplier Abbkine

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