Rabbit INS (Insulin) ELISA Kit

ELISA, Rabbit ELISA, Elabscience

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Product Name Rabbit INS (Insulin) ELISA Kit
Species Rabbit
Description This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit INS . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit INS. The concentration of Rabbit INS in samples can be calculated by comparing the OD of the samples to the standard curve.
Synonyms Insulin
Method Sandwich ELISA
Detection Range 3.125-200ng/mL
Sensitivity 1.875ng/mL
Sample Types Serum, Plasma, Cell supernatant
Uniprot No. P01311
Intended Use For research use only, not for use in diagnostic procedures.

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