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|Product Name||RAP1A, 1-181aa, Human|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS KINVNEIFYD LVRQINRKTP VEKKKPKKKS C|
|Background||Ras-related protein Rap-1A (RAP1A) belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. This Protein Induces morphological reversion of a cell line transformed by a Ras oncogene and Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Recombinant human RAP1A, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol|
|Intended Use||For Research Use Only|
|Molecular Weight||14.8 kDa(138aa), confirmed by MALDI-TOF|
|NCBI Accession #||NP_001010935|
|Purity||> 85% by SDS-PAGE|
|References||Han, L., et al. (1995) Mol Cell Biol. 15 (3): 1318-23.
Rebhun, J F, et al. (2000) J Biol Chem. 275 (45): 34901-8.
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||KREV1, G-22K, C21KG, Ras-related protein Rap-1A, G 22K, GTP binding protein smg p21A, KREV 1, OTTHUMP00000013741, RAP 1A, RAP1, RAP1A member of RAS oncogene family, Ras related protein Krev 1, Ras related protein Rap 1A, RAS related protein RAP1A, SMGP21|
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