Rat Slit homolog 1 protein (SLIT1) ELISA Kit

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KTE100241
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Product Name Rat Slit homolog 1 protein (SLIT1) ELISA Kit
Description Rat Slit homolog 1 protein (SLIT1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SLIT1. No significant cross-reactivity or interference between Rat SLIT1 and analogues was observed. The SLIT1 cDNA encodes a 1,534-amino acid polypeptide with 43.5% similarity to the Drosophila 'slit' protein. Northern blot analysis revealed that the human SLIT1 gene was expressed as a major 8.4- and a minor 5.9-kb transcript primarily in the brain. SLIT2 and SLIT3 mRNAs were primarily expressed in the spinal cord and thyroid, respectively. In situ hybridization studies indicated that the rat Slit1 mRNA was specifically expressed in the neurons of fetal and adult forebrains. These data suggested that the SLIT genes form an evolutionarily conserved group in vertebrates and invertebrates, and that the mammalian SLIT proteins may participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions. This Rat Slit homolog 1 protein (SLIT1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLIT1 in samples. An antibody specific for SLIT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLIT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLIT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLIT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Synonyms SLIT1, RP11-175O19.2, MEGF4, MGC164811, SLIL1, SLIT3, Slit-1, slit homolog 1
Method Sandwich ELISA
Detection Range Please inquire
Sensitivity Please inquire
Reactivity Rat
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene Id 65047
Storage 2-8°C
Supplier Abbkine

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