S100A3, 1-101aa, Human

Proteins, Recombinant
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Product Name S100A3, 1-101aa, Human
Applications SDS-PAGE
Background S100A3 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A3 binds calcium with low affinity and zinc with high affinity and is believed to play a role in the calcium-dependent differentiation of cuticle cells and in the formation of hair shafts. Recombinant human S100A3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Concentration 0.5mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 7.5) containing 2mM DTT, 20% glycerol, 100mM NaCl
Species Human
Host E.coli
Intended Use For Research Use Only
Molecular Weight 24.2kDa (222aa), confirmed by MALDI-TOF
NCBI Accession # NP_002951
Purity > 90% by SDS-PAGE
References Fritz G., et al. (1998) Biochim Biophys Acta. 1448(2):264-76.
Kizawa K., et al. (2008) J Biol Chem. 283(8):5004-13.
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Synonyms S100 calcium binding protein A3, S100E
Tag His-tag

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