| Product Name | Sheep Adiponectin (ADIPOQ) ELISA Kit |
|---|---|
| Description | Adiponectin, alternatively named Adipocyte Complement-Related Protein of 30 kDa, shares structural similarity with complement factor C1q and is a member of the family of defense collagens. It is secreted exclusively by differentiated adipocytes and circulates at high concentrations. Adiponectin has a modular structure comprising an N-terminal collagenous domain with multiple collagen triple helix repeats, followed by a C-terminal C1q-like globular domain. The globular domain has similar folding topology with tumor necrosis factor-a and assembles into homotrimers. Higher order oligomeric adiponectins are also formed via interactions between the collagenous stalk. A truncated form of Adiponectin containing only the globular domain can be generated by proteolytic cleavage. Sheep Adiponectin (ADIPOQ) ELISA Kit employs a two-site sandwich ELISA to quantitate Adiponectin in samples. An antibody specific for Adiponectin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Adiponectin present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Sheep Adiponectin detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of Adiponectin bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | ADIPOQ |
| Method | Sandwich ELISA |
| Detection Range | 7.5 μg/L-120 μg/L |
| Sensitivity | 0.5 μg/L |
| Reactivity | Sheep |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 282865 |
| Storage | 2-8°C |
| Supplier | Abbkine |
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