Sheep Cytochrome c (CYCS) ELISA Kit

Category: Assay Kits
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KTE110053
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Product Name Sheep Cytochrome c (CYCS) ELISA Kit
Description Sheep Cytochrome c (CYCS) ELISA Kit has high sensitivity and excellent specificity for detection of Sheep CYCS. No significant cross-reactivity or interference between Sheep CYCS and analogues was observed. Cytochrome c (CYCS) gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. This Sheep Cytochrome c (CYCS) ELISA Kit employs a two-site sandwich ELISA to quantitate CYCS in samples. An antibody specific for CYCS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYCS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYCS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYCS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Synonyms CYCS, CYC, HCS, THC4, OTTHUMP00000202043, OTTHUMP00000202044, cytochrome c
Method Sandwich ELISA
Detection Range Please inquire
Sensitivity Please inquire
Reactivity Sheep
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene Id 106990092
Storage 2-8°C
Supplier Abbkine

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