| Product Name | SMAC/Diablo, 56-239aa, Human, T7 tagged, Recombinant |
|---|---|
| Description | Smac/Diablo is one of the proapoptotic proteins. Smac promotes caspase activation in the cytochrome c/Apaf-1/caspase-9 pathway by binding to inhibitor of apoptosis proteins (IAPs) and removing their inhibitory activity. Recombinant human Smac fused to T7-tag. at N-terminus was expressed in E.coli and purified by conventional chromatography techniques. |
| Synonyms | SMAC3, DIABLO-S, FLJ10537, FLJ25049, Diablo isoform 1, SMAC/Diablo, Diablo isoform 1 0610041G12Rik, DBOH, Diablo homolog, DIABLO S, Diablo homolog (Drosophila), Mitochondrial Smac protein, Diablo homolog mitochondrial, SMAC 3, Smac protein, Direct IAP binding protein with low pI, Second mitochondria derived activator of caspase. |
| Host | E.coli |
| Molecular Weight | 40.7 kDa (366 aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MASMTGGQQM GRGSMAVPIA QKSEPHSLSS EALMRRAVSL VTDSTSTFLS QTTYALIEAI TEYTKAVYTL TSLYRQYTSL LGKMNSEEED EVWQVIIGAR AEMTSKHQEY LKLETTWMTA VGLSEMAAEA AYQTGADQAS ITARNHIQLV KLQVEEVHQL SRKAETKLAE AQIEELRQKT QEEGEERAES EQEAYLRED |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 50 mM Tris-HCl buffer (pH7.5) 0.2M NaCl, 5mM DTT, 20%glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Srinivasula SM., et al. (2000). J Biol Chem. 275(46):36152-7. DU C., et al. (2000). Cell. 102(1):33-42. |
| Supplier | ARP |
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