SNAP-25 (Synaptosomal-associated protein 25kDa), C.elegans Recombinant

Proteins, Recombinant
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Product Name SNAP-25 (Synaptosomal-associated protein 25kDa), C.elegans Recombinant
Applications SDS-PAGE
Background SNAP-25 is a presynaptic plasma membrane protein essential for synaptic vesicle fusion and neurotransmitter release . The membrane proteins SNAP-25, synaptobrevin and syntaxin make up the core complex (SNARE) essential for docking and holding synaptic vesicles at the pre synaptic membrane. SNAP-25 gene was amplified by PCR from C.elegans and cloned into an E. coli expression vector. SNAP-25 was purified by using conventional chromatography techniques.
Concentration 1 mg/ml (determined by BCA assay)
Form Liquid, in 20 mM Tris-HCl buffer (pH 7.5) containing 0.1 M NaCl
Species Human
Host E.coli
Intended Use For Research Use Only
Molecular Weight 13.3 kDa (127 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
NCBI Accession # NP_003072.2
Purity > 95% by SDS-PAGE
References Saifee O., et al. (1998) Mol.Biol.Cell 9(6);1235-52.
Yang Y., et al. (2000) . 275(38);29482-7.
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Synonyms SNAP25, SNAP-25, Synaptosomal-associated protein 25kDa, FLJ23079, RIC-4, RIC4, SEC9, SNAP 25, bA416N4.2, dJ1068F16.2, SUP, resistance to inhibitors of cholinesterase 4 homolog, super protein, synaptosomal-associated 25 kDa protein, synaptosomal-associated protein 25, RIC 4
Tag His-tag

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