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|Product Name||14-3-3 beta, 1-246aa, Human, Recombinant|
|Background||The 14-3-3 family plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 beta, a subtype of the 14-3-3 proteins, was found in B Cells, brain and liver etc. This 14-3-3 beta has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Recombinant human 14-3-3 beta was expressed in E.coli and purified by using conventional chromatography techniques.|
|Synonyms||Tyrosine 3-monocxygenase/tryptopan 5-monooxygenase activation protein, beta polypeptide, 14-3-3 beta, YWHAB, GW128, HS1, KCIP-1, Tyrosine 3-monocxygenase/tryptopan 5-monooxygenase activation protein, beta polypeptide 14 3 3 protein beta/alpha, Brain protein 14 3 3 beta isoform, GW 128, HS 1, KCIP 1, KCIP1, Protein 1054, Protein kinase C inhibitor protein 1, Tyrosine 3 Monooxygenase/Tryptophan 5 Monooxygenase Activation Protein Beta Polypeptide.|
|Amino Acid Sequence||MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY KNVVGARRSS WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICNDVL ELLDKYLIPN ATQPESKVFY LKMKGDYFRY LSEVASGDNK QTTVSNSQQA YQEAFEISKK EMQPTHPIRL GLALNFSVFY YEILNSPEKA CSLAKTAFDE AIAELDTLNE ESYKDSTLIM QLLRDNLTLW TSENQGDEGD AGEGEN|
|Molecular Weight||16.3 kDa (142aa), confirmed by MALDI-TOF.|
|Concentration||0.5 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris pH 8.0|
|Purity||> 90% by SDS-PAGE|
|NCBI Accession #||NP_003395|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Rodriguez LG., et al. (2005) J Cell Physiol. 202(1):285-94.
Mils V., et al. (2000) Oncogene. 19(10):1257-65.
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