Acid phosphatase 1, 1-158aa, Human, Recombinant

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01-2129
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Proteins, Recombinant
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Product Name Acid phosphatase 1, 1-158aa, Human, Recombinant
Background Acid phosphatase 1, soluble, also known as ACP1, belongs to the phosphotyrosine protein. It functions as an acid phosphatase and a protein tyrosine phosphatase (PTPase) present in all human tissues, including adipocytes. This enzyme hydrolyzes protein tyrosine phosphate to protein tyrosine and orthophosphate, and also orthophosphoric monoesters to alcohol and orthophosphate. Recombinant ACP1, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.
Synonyms Acid phosphatase 1, soluble isoform b, ACP1, HAAP, LMW-PTP, Red cell acid phosphatase 1, Adipocyte acid phosphatase, Acid phosphatase 1, soluble isoform b Acid phosphatase 1 soluble, Adipocyte acid phosphatase Cytoplasmic phosphotyrosyl protein phosphatase, Low molecular weight phosphotyrosine protein phosphatase, Acid phosphatase of erythrocyte, PAP2, Protein tyrosine phosphatase, PTPase, Purple acid phosphatase, Red cell acid phosphatase 1.
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENW VIDSGAVSDW NVGRSPDPRA VSCLRNHGIH TAHKARQITK EDFATFDYIL CMDESNLRDL NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAH
Endotoxin Level < 1.0 EU per 1 microgram of protein (determined by LAL method)
Molecular Weight 27.7 kDa (250aa), confirmed by MALDI-TOF.
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_009030
Host E.coli
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References De Lorenzo A., et al. (2009) Metabolism.58(3):351-4
Banci M., et al. (2009) Cardiology.113(4):236-242.

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