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|Product Name||AKR1C3, 1-323aa, Human, Recombinant|
|Background||AKR1C3, also known as PGFS, is a member of the aldo-keto reductase superfamily which catalyzes the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9 alpha, 11 beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and differentiation. Recombinant human AKR1C3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Synonyms||Aldo-keto reductase family 1, member C3, DD3, DDX, HA1753, HAKRB, HAKRe, hluPGFS, HSD17B5, KIAA0119, Aldo-keto reductase family 1, member C3 17 beta HSD 5, AKR1 C3, Chlordecone reductase, 17 beta hydroxysteroid dehydrogenase type 5, Aldo keto reductase family 1 member C3, DDH1, HAKRe, HSD17B5, PGFS, Prostaglandin F synthase,|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MDSKHQCVKL NDGHFMPVLG FGTYAPPEVP RSKALEVTKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWSTFH RPELVRPALE NSLKKAQLDY VDLYLIHSPM SLKPGEELSP TDENGKVIFD IVDLCTTWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNRS KLLDFCKSKD IVLVAYSALG SQRDKRWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTA EDMKAIDGLD RNLHYFNSDS FASHPNYPYS DEY|
|Endotoxin Level||< 1.0 EU per 1 microgram of protein (determined by LAL method)|
|Molecular Weight||35.8kDa (316aa), confirmed by MALDI-TOF.|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol|
|Purity||> 95% by SDS-PAGE|
|NCBI Accession #||NP_003730|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Davies N., et al. (2009) Cancer Res. 69(11):4769-75
Kabututu Z., et al. (2009) J Biochem. 145(2):161-8
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