ANGPT1, 20-498aa, Human

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01-P1236
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Proteins, Recombinant
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Product Name ANGPT1, 20-498aa, Human
Background Human ANGPT1 is a secreted ligand for Tie-2, a cell surface receptor tyrosine kinase expressed in endothelial and hemopoietic cells. This protein is glycosylated and has a fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. ANGPT1 is an angiogenic factor that mediates blood vessel maturation and may be involved in endothelial development. Recombinant human ANGPT1 protein was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Angiopoietin 1, AGP1, AGPT, ANG1
Species Human
Amino Acid Sequence MSNQRRSPEN SGRRYNRIQH GQCAYTFILP EHDGNCREST TDQYNTNALQ RDAPHVEPDF SSQKLQHLEH VMENYTQWLQ KLENYIVENM KSEMAQIQQN AVQNHTATML EIGTSLLSQT AEQTRKLTDV ETQVLNQTSR LEIQLLENSL STYKLEKQLL QQTNEILKIH EKNSLLEHKI LEMEGKHKEE LDTLKEEKEN LQGLVTRQTY IIQELEKQLN RATTNNSVLQ KQQLELMDTV HNLVNLCTKE GVLLKGGKRE EEKPFRDCAD VYQAGFNKSG IYTIYINNMP EPKKVFCNMD VNGGGWTVIQ HREDGSLDFQ RGWKEYKMGF GNPSGEYWLG NEFIFAITSQ RQYMLRIELM DWEGNRAYSQ YDRFHIGNEK QNYRLYLKGH TGTAGKQSSL ILHGADFSTK DADNDNCMCK CALMLTGGWW FDACGPSNLN GMFYTAGQNH GKLNGIKWHY FKGPSYSLRS TTMMIRPLDF
Molecular Weight 28.0 kDa (254aa), confirmed by MALDI-TOF
Concentration 1.0 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 30% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF
Purity > 85 % by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_001137
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Kopczynska E., et al. (2009) Eur J Gynaecol Oncol. 30(6):646-9.
Davis S., et al. (1996) Cell. 87(7):1161-9.

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