Anti-Adipophilin / ADRP Monoclonal Antibody

Category: Antibodies
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03-610102
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Product Name Anti-Adipophilin / ADRP Monoclonal Antibody
Description The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. Mouse Monoclonal Antibody to Human homologue to mouse adipocyte differentiation-related protein (ADRP). Adipophilin / ADRP is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines (see e.g. Heid et al.; for review see e.g. Targett- Adams et al.); inducible by etomoxir. Enhanced expression of Adipophilin / ADRP is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study virus entry via lipid droplets (see e.g. Hope et al., Samsa et al.). Polypeptide reacting: Adipophilin / ADRP, MW 48,100 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE); pI 6.72.
Synonyms ADFP, ADRP
Clone AP 125
Immunogen Synthetic peptide (aa 5-27 from N-terminus of human adipophilin)
Isotype IgG1
Specificity Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative. Also positively stained are lipid-storing CD 68-positive macrophages.
Reactivity Canine, Human, Mouse, Rat
Applications IHC, WB
Form Affinity purified, 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4
Storage 2-8C for immediate use, or at -20C (aliquot)
References Heid, H.W.et al. (1997) (submitted for publication)Heid, HWet al. (1996) Biochem J. 320: 1025-1030*Steiner, S. et al. (1996). Biochem. Biophys. Res. 218: 777-778Mrozinzki, J et al. (1999) J. Cancer Res&Clin. Oncol. 125 supplementThan, NG et al. (1999) Tumor Biol. 20: 184-192Hope, RG et al. (2000) J. General Virol. 81: 1913-1925Ostermeyer, AG. Et al.(2001) J. Cell Biol. 152: 1071-1078Fujimoto, T. et al. (2001) j. Cell Biol. 152: 1079-1085Buechler, C. et al. (2001) Biochim Biophys Acta 1532: 97-104
Supplier ARP

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