| Product Name | Anti-Bromodeoxyuridine (BrdU) Monoclonal Antibody, FITC |
|---|---|
| Description | Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. It is a valuable tool for detection of S-phase (DNA- synthesizing cells) in cell ad tissue culture. 3H-thymidine labelling techniques can be avoided. Contains Evans Blue counterstain. |
| Clone | BU5.2 |
| Immunogen | Bromodeoxyuridine |
| Isotype | IgG2a |
| Specificity | This FITC-labelled antibody is specific for bromodesoxyuridine and does not cross-react with thymidine |
| Form | Purified (Protein A), in PBS containing 1% BSA and 0.1% sodium-azide |
| Storage | 2-8C |
| Notes | Detection of bromodeoxyuridine incorporation in tissue and cell culture (inquire the protocol) |
| References | Hosihno, T. et al. (1985) Cytometrie 6: 627-632 |
| Supplier | ARP |
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