| Product Name | Anti-Synaptopodin Monoclonal Antibody |
|---|---|
| Description | Synaptopodin is an actin-associated protein that may play a role in actin-based cell shape and motility. The name synaptopodin derives from the protein's associations with postsynaptic densities and dendritic spines and with renal podocytes (Mundel et al., 1997 [PubMed 9314539]). This antibody recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin. Does not react with parietal cells. It reacts with a subset of exclusively telencephalic synapses. Differentiation- dependent expression during postnatal maturation of rat brain. Differentiation- dependent expression in cultured hippocampal neurons. |
| Synonyms | SYNPO |
| Clone | G1D4 |
| Immunogen | Isolated rat kidney glomeruli |
| Isotype | IgG1 |
| Specificity | The antibody reacts specifically with synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin. Synaptopodin represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications). In Western blot analysis the antibody also reacts with a 44 kD degradation fragment of synaptopodin |
| Reactivity | Amphibian, Gallus, Rodent, Bovine, Guinea Pig, Human, Mouse, Rat |
| Applications | IF, IHC, WB |
| Form | Purified (Protein A), in PBS containing 1% BSA and 0.1% sodium-azide |
| Storage | 2-8C for immediate use, or at -20C (aliquot) |
| References | 1. Mundel, P. et al. (1991) J. Histochem & Cytochem 39 No.8: 1047-1056 2. Mundel, P. & Kritz, W. (1995) Anat. Embryol. 192: 385-397 3. Mundel, P. et al. J Am Soc Nephrol 8: 8978-705 4. Mundel, P. et al. (1997) J. Cell Biol. 139(1): 193-204 5. Mundel, P. et al. (1997) Exp. Cell Res 236: 248-258 6. Kobayashi N et al. (1998) J Cell Biol 143: 1961-1970 7. Barisoni L et al. (1999) J Am Soc Nephol 10: 51-61 8. Kihara I et a;\l (1999) Histopathology 34(6): 537-547 9. Asanuma K et al. (2005) J Clin Invest 115: 1188-1198 |
| Supplier | ARP |
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