Antigen 85A, 43-338aa, Mycobacterium tuberculosis, Baculovirus

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01-P1490
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Proteins, Recombinant
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Product Name Antigen 85A, 43-338aa, Mycobacterium tuberculosis, Baculovirus
Background Antigen 85A, belong to the antigen 85 complex (Antigen 85A, B, C). The enzymes of the antigen 85 complex possess mycolyltransferase activity and catalyze the synthesis of the most abundant glycolipid of the mycobacterial cell wall, the cord factor. The cord factor (trehalose 6,6'-dimycolate, TDM) is essential for the integrity of the mycobacterial cell wall and pathogenesis of the bacillus. TDM is synthesized from two molecules of trehalose-6'-monomycolate (TMM) by Antigen 85A. Recombinant Mycobacterium tuberculosis Antigen 85A protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell and purified by using conventional chromatography techniques.
Synonyms Antigen 85 complex A, Mycolytransferase 85A, Fibronectin-binding protein A, TB Ab85A, TBab85A
Species Mycobacterium tuberculosis
Amino Acid Sequence ADPAFSRPGL PVEYLQVPSP SMGRDIKVQF QSGGANSPAL YLLDGLRAQD DFSGWDINTP AFEWYDQSGL SVVMPVGGQS SFYSDWYQPA CGKAGCQTYK WETFLTSELP GWLQANRHVK PTGSAVVGLS MAASSALTLA IYHPQQFVYA GAMSGLLDPS QAMGPTLIGL AMGDAGGYKA SDMWGPKEDP AWQRNDPLLN VGKLIANNTR VWVYCGNGKP SDLGGNNLPA KFLEGFVRTS NIKFQDAYNA GGGHNGVFDF PDSGTHSWEY WGAQLNAMKP DLQRALGATP NTGPAPQGAH HHHHH
Molecular Weight 21.3 kDa (198aa)
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.4M Urea
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_218321.1
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Elamin AA., et al. (2009) J Microbiol Methods. 79(3):358-63.
Jeon BY., et al. (2011) Microbes Infect. 13(3):284-90.

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