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|Product Name||ARF4, 1-180aa, Human|
|Background||ARF4 is a member of the ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The ARF proteins include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. They are categorized as class I, class II and class III; this gene is a class II member. Recombinant human ARF4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Synonyms||ADP-ribosylation factor 4, ARF2|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MGSHMGLTIS SLFSRLFGKK QMRILMVGLD AAGKTTILYK LKLGEIVTTI PTIGFNVETV EYKNICFTVW DVGGQDRIRP LWKHYFQNTQ GLIFVVDSND RERIQEVADE LQKMLLVDEL RDAVLLLFAN KQDLPNAMAI SEMTDKLGLQ SLRNRTWYVQ ATCATQGTGL YEGLDWLSNE LSKR|
|Molecular Weight||38.5 kDa (349aa), confirmed by MALDI-TOF|
|Concentration||0.25 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 30% glycerol|
|Purity||> 95% by SDS-PAGE|
|NCBI Accession #||NP_001651|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Mazelova J., et al. (2009) EMBO J. 28(3):183-92.
Woo IS., et al. (2009) Cancer Lett. 276(1):53-60.
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