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|Product Name||ASPH, 75-270aa, Human|
|Background||ASPH (Aspartate beta-hydroxylase) is a widely-expressed type II membrane protein involved in calcium homeostasis. Located in the endoplasmic reticulum, ASPH specifically hydroxylates an Asp or Asn residue in the epidermal growth factor-like (EGF) domains of several proteins, using iron as a cofactor. While all ASPH variants are expressed in skeletal muscle, only some are detected in heart, brain, pancreas, placenta, lung, liver, and kidney tissues. Recombinant human ASPH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Synonyms||AAH, BAH, CASQ2BP1, HAAH, JCTN, junctin, Aspartyl/asparaginyl beta-hydroxylase ASP beta hydroxylase, Aspartyl/asparaginyl beta hydroxylase, Peptide aspartate beta dioxygenase,|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MFDLVDYEEV LGKLGIYDAD GDGDFDVDDA KVLLGLKERS TSEPAVPPEE AEPHTEPEEQ VPVEAEPQNI EDEAKEQIQS LLHEMVHAEH ETEHSYHVEE TVSQDCNQDM EEMMSEQENP DSSEPVVEDE RLHHDTDDVT YQVYEEQAVY EPLENEGIEI TEVTAPPEDN PVEDSQVIVE EVSIFPVEEQ QEVPPDT|
|Molecular Weight||23.9kDa (205aa), confirmed by MALDI-TOF.|
|Concentration||0.25 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH7.5) containing 20% glycerol, 100mM NaCl, 0.1mM PMSF|
|Purity||> 90% by SDS-PAGE|
|NCBI Accession #||NP_001158227|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Kwon SJ., et al. (2009) Biochem Biophys Res Commun. 390(4):1389-94.|
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