ATF4, 1-351aa, Human, His-Cam tag

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01-ATF-4
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Proteins, Recombinant
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Product Name ATF4, 1-351aa, Human, His-Cam tag
Background Activating transcription factor 4, also known as ATF4, belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins and CREB-like proteins. ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. Recombinant human ATF4 protein, fused to His-Calmodulin tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Activating transcription factor 4, CREB2, TXREB, CREB-2
Species Human
Amino Acid Sequence MHHHHHHMAD QLTEEQIAEF KEAFSLFDKD GDGTITTKEL GTVMRSLGQN PTEAELQDMI NEVDADGNGT IDFPEFLTMM ARKMKDTDSE EEIREAFRVF DKDGNGYISA AELRHVMTNL GEKLTDEEVD EMIREADIDG DGQVNYEEFV QMMTAKGSHM TEMSFLSSEV LVGDLMSPFD QSGLGAEESL GLLDDYLEVA KHFKPHGFSS DKAKAGSSEW LAVDGLVSPS NNSKEDAFSG TDWMLEKMDL KEFDLDALLG IDDLETMPDD LLTTLDDTCD LFAPLVQETN KQPPQTVNPI GHLPESLTKP DQVAPFTFLQ PLPLSPGVLS STPDHSFSLE LGSEVDITEG DRKPDYTAYV AMIPQCIKEE DTPSDNDSGI CMSPESYLGS PQHSPSTRGS PNRSLPSPGV LCGSARPKPY DPPGEKMVAA KVKGEKLDKK LKKMEQNKTA ATRYRQKKRA EQEALTGECK ELEKKNEALK ERADSLAKEI QYLKDLIEEV RKARGKKRVP
Molecular Weight 22kDa (189aa), confirmed by MALDI-TOF
Concentration 0.5mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 200mM NaCl
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_001666
Host E.coli
Tag T7 tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Lin Y S., et al. (1988) Proc Natl Acad Sci. 85:3396-4000.
Hoeffler JP., et al. (1988) Science. 242:1430-1433.

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