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|Product Name||ATP5D, 23-168aa, Human|
|Background||ATP5D, also known as F-ATPase delta subunit, catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This protein is the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identified. Recombinant human ATP5D protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.|
|Synonyms||ATP synthase subunit delta, mitochondrial, F-ATPase delta subunit|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MAEAAAAPAA ASGPNQMSFT FASPTQVFFN GANVRQVDVP TLTGAFGILA HVPTLQVLR PGLVVVHAED GTTSKYFVSS GSIAVNADSS VQLLAEEAVT LDMLDLGAAK ANLEKAQAEL VGTADEATRA EIQIRIEANE ALVKALE|
|Molecular Weight||28.1 kDa (248aa), confirmed by MALDI-TOF|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.1M NaCl, 1mM DTT|
|Purity||> 95% by SDS-PAGE|
|NCBI Accession #||NP_001678|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Jordan E.M., et al. (1992) Biochim. Biophys. Acta 1130:123-126
Grimwood J., et al. (2004) Nature. 428:529-535
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