ATP5O, 24-213aa, Human

Category: Proteins
Catalog
01-1830-O
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Product Name ATP5O, 24-213aa, Human
Description ATP synthase subunit O, also known as ATP5O, localizes to the mitochondria and catalyzes ATP synthesis. The protein is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance. Recombinant human ATP5O protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms ATP synthase subunit O, ATPO, OSCP
Host E.coli
Molecular Weight 16.7kDa (154aa), confirmed by MALDI-TOF
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MFAKLVRPPV QVYGIEGRYA TALYSAASKQ NKLEQVEKEL LRVAQILKEP KVAASVLNPY VKRSIKVKSL NDITAKERFS PLTTNLINLL AENGRLSNTQ GVVSAFSTMM SVHRGEVPCT VTSASPLEEA TLSELKTVLK SFLSQGQVLK LEAKTDPSIL GGMIVRIGEK YVDMSVKTKI QKLGRAMREI V
Tag His-tag
Reactivity Human
Applications SDS-PAGE
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 2mM DTT, 100mM NaCl
Concentration 0.5mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
References Hundal T., et al. (1984) J Bioenerq Biomembr. 16:535-550.
Joshi S., et al. (1992) J Biol Chem. 267:12860-12867.
Supplier ARP

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