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|Product Name||BHMT (Betaine-homocysteine methyltransferase), Human, Recombinant|
|Background||Betaine-homocysteine methyltransferase (BHMT) is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. BHMT displays differential expression in a model of liver cirrhosis. Recombinant human BHMT, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Synonyms||BHMT, BHMT (Betaine-homocysteine methyltransferase), EC 18.104.22.168, Betaine--homocysteine S-methyltransferase 1, Betaine-homocysteine methyltransferase Betaine homocysteine methyltransferase, Betaine homocysteine S methyltransferase, Betaine homocysteine S methyltransferase 1, BHMT1,|
|Amino Acid Sequence||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMPP VGGKKAKKGI LERLNAGEIV IGDGGFVFAL EKRGYVKAGP WTPEAAVEHP EAVRQLHREF LRAGSNVMQT FTFYASEDKL ENRGNYVLEK ISGQEVNEAA CDIARQVADE GDALVAGGVS QTPSYLSCKS ETEVKKVFLQ QLEVFMKKNV DFLIAEYFEH VEEAVWAVET LIASGKPVAA TMCIGPEGDL HGVPPGECAV RLVKAGASII GVNCHFDPTI SLKTVKLMKE GLEAARLKAH LMSQPLAYHT PDCNKQGFID LPEFPFGLEP RVATRWDIQK YAREAYNLGV RYIGGCCGFE PYHIRAIAEE LAPERGFLPP ASEKHGSWGS GLDMHTKPWV RARARKEYWE NLRIASGRPY NPSMSKPDGW GVTKGTAELM QQKEATTEQQ LKELFEKQKF KSQ|
|Molecular Weight||28.4 kDa (256aa), confirmed by MALDI-TOF.|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Form||Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 1mM DTT,10% glycerol|
|Purity||> 95% by SDS-PAGE|
|NCBI Accession #||NP_001704|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Nandita, et al., Protein Expr Purif (2002) 25:73-80
Breksa, A P et al., Biochemistry (1999) 38:13991-8
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