| Product Name | BMP-2 (active), 283-396aa, Human |
|---|---|
| Description | BMP-2 (Bone morphogenetic protein 2) is multi-functional growth factor that belongs to the transforming growth factor beta (TGF beta) superfamily. It plays an important role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration. BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility. Recombinant human BMP-2 protein was expressed in E.coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer. |
| Synonyms | Bone morphogenetic protein 2, BMP-2A, bmp2, Bone morphogenetic protein-2 |
| Host | E.coli |
| Molecular Weight | 44.3kDa (398aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20 mM Tris-HCl buffer, pH8.0, 10% glycerol, 1mM DTT, 50mM NaCl |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Chen D., et al. (2004). Growth factors. 22(4):233-41 Schliephake H., et al. (2005). Clin Oral Implants Res. 17(6):666-72 |
| Supplier | ARP |
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