Product Name | Carbonic anhydrase 1, 1-261aa, Human |
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Description | Carbonic anhydrase 1(CA1) is a zinc metalloenzyme that catalyses reversible hydration of CO2 (CO2 + H2O <-> HCO3- + H+). This protein is fundamental to many biological processes such as cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, salvia, and gastric acid. It is most abundant in erythrocytes and is a very early marker for erythroid differentiation. Recombinant human Carbonic anhydrase1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
Synonyms | CA1, CA-I, CAB, JW0122, CA 1, CA I, CAI, CAN, Car 1, Car1, Carbonate dehydratase I, Carbonic anhydrase 1, Carbonic anhydrase B, Carbonic dehydratase, ECK0125, yadF, |
Host | E.coli |
Molecular Weight | 16.4 kDa (142aa), confirmed by MALDI-TOF. |
Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F |
Reactivity | Human |
Applications | SDS-PAGE |
Form | Liquid, in 50mM Tris-HCl buffer (pH7.5) containing 10% glycerol |
Concentration | 1 mg/ml (determined by Bradford assay) |
Purity | > 95% by SDS-PAGE |
Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
References |
Nogradi A., et al. (1998). Am J Pathol. 153:1-4 Ferry JG., et al. (1999). Proc Natl Acad Sci U S A. 96(26):15184-9. |
Supplier | ARP |
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