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|Product Name||CCNA2, 1-432aa, Human|
|Background||CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Recombinant human CCNA2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Synonyms||Cyclin A2, CCN1, CCNA|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MGSHMLGNSA PGPATREAGS ALLALQQTAL QEDQENINPE KAAPVQQPRT RAALAVLKSG NPRGLAQQQR PKTRRVAPLK DLPVNDEHVT VPPWKANSKQ PAFTIHVDEA EKEAQKKPAE SQKIEREDAL AFNSAISLPG PRKPLVPLDY PMDGSFESPH TMDMSIVLED EKPVSVNEVP DYHEDIHTYL REMEVKCKPK VGYMKKQPDI TNSMRAILVD WLVEVGEEYK LQNETLHLAV NYIDRFLSSM SVLRGKLQLV GTAAMLLASK FEEIYPPEVA EFVYITDDTY TKKQVLRMEH LVLKVLTFDL AAPTVNQFLT QYFLHQQPAN CKVESLAMFL GELSLIDADP YLKYLPSVIA GAAFHLALYT VTGQSWPESL IRKTGYTLES LKPCLMDLHQ TYLKAPQHAQ QSIREKYKNS KYHGVSLLNP PETLNL|
|Molecular Weight||25.6kDa (222aa), confirmed by MALDI-TOF|
|Concentration||0.5mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20 mM Tris-HCl buffer, pH8.0, 10% glycerol, 2mM DTT, 200mM NaCl|
|Purity||> 90% by SDS-PAGE|
|NCBI Accession #||NP_001228|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Alvarez-Fernandez,M., et al. (2011) J. Biol. Chem. 286 (38), 33029-33036
Kim,D.H., et al. (2011) Cancer 117 (17), 4080-4091
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