| Product Name | CDC26, 1-85aa, Human |
|---|---|
| Description | CDC26, also known as ANAPC12, APC12, belongs to the CDC26 family which orchestrates progression through mitosis by decorating cell-cycle regulators with ubiquitin chains. This protein is component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins. Recombinant human CDC26 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Synonyms | Anaphase-promoting complex subunit CDC26, ANAPC12, APC12 |
| Host | E.coli |
| Molecular Weight | 21.1kDa (185aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MLRRKPTRLE LKLDDIEEFE NIRKDLETRK KQKEDVEVVG GSDGEGAIGL SSDPKSREQM INDRIGYKPQ PKPNNRSSQF GSLEF |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 100mM NaCl |
| Concentration | 0.5mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Jin L., et al. (2008) Cell. 133:653-665 Gmachl M., et al. (2000) Proc. Natl. Acad. Sci. U.S.A. 97:8973-8978 |
| Supplier | ARP |
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