CHIP, 1-303aa, Human, Recombinant

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01-2047
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Proteins, Recombinant
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Product Name CHIP, 1-303aa, Human, Recombinant
Background CHIP, also known as STUB1, is a cytoplasmic protein whose amino acid sequence is highly conserved across species. CHIP interacts with the molecular chaperones Hsc70-Hsp70 and Hsp90 through its TPR domain, whereas its U-box domain contains its E3 ubiquitin ligase activity. Its interaction with these molecular chaperones results in client substrate ubiquitylation and degradation by the proteasome. Thus, CHIP acts to tilt the folding-refolding machinery toward the degradative pathway, and it serves as a link between the two. Recombinant human CHIP protein was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms STUB1, HSPABP2, NY-CO-7, SDCCAG7, UBOX1, Carboxy terminus of HSP70 interacting protein Antigen NY CO 7, CLL associated antigen KW 8, Carboxy terminus of Hsp70p interacting protein, CHIP, E3 ubiquitin protein ligase CHIP, STUB 1, UBOX 1, Heat shock protein A binding protein 2 (c terminal), NY CO 7, Serologically defined colon cancer antigen 7, STIP1 homology and U Box containing protein 1.
Species Human
Amino Acid Sequence MKGKEEKEGG ARLGAGGGSP EKSPSAQELK EQGNRLFVGR KYPEAAACYG RAITRNPLVA VYYTNRALCY LKMQQHEQAL ADCRRALELD GQSVKAHFFL GQCQLEMESY DEAIANLQRA YSLAKEQRLN FGDDIPSALR IAKKKRWNSI EERRIHQESE LHSYLSRLIA AERERELEEC QRNHEGDEDD SHVRAQQACI EAKHDKYMAD MDELFSQVDE KRKKRDIPDY LCGKISFELM REPCITPSGI TYDRKDIEEH LQRVGHFDPV TRSPLTQEQL IPNLAMKEVI DAFISENGWV EDY
Endotoxin Level < 1.0 EU per 1 microgram of protein (determined by LAL method)
Molecular Weight 27kDa (240aa), confirmed by MALDI-TOF.
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris pH8.0, 1mM DTT
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_005852
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Kajiro M., et al. (2009) Nat Cell Biol. 11(3):312-9.
Ko HS., et al. (2009) Proc Natl Acad Sci. 106(8):2897-902.

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