CIAO1, 1-339aa, Human

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01-P2006
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Proteins, Recombinant
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Product Name CIAO1, 1-339aa, Human
Background CIAO1 is essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. This protein is required for the maturation of extra mitochondrial Fe/S proteins and seems to specifically modulate the trans-activation activity of WT1. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation. Recombinant human CIAO1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Probable cytosolic iron-sulfur protein assembly protein CIAO1, CIA1, WDR39
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MGSMKDSLVL LGRVPAHPDS RCWFLAWNPA GTLLASCGGD RRIRIWGTEG DSWICKSVLS EGHQRTVRKV AWSPCGNYLA SASFDATTCI WKKNQDDFEC VTTLEGHENE VKSVAWAPSG NLLATCSRDK SVWVWEVDEE DEYECVSVLN SHTQDVKHVV WHPSQELLAS ASYDDTVKLY REEEDDWVCC ATLEGHESTV WSLAFDPSGQ RLASCSDDRT VRIWRQYLPG NEQGVACSGS DPSWKCICTL SGFHSRTIYD IAWCQLTGAL ATACGDDAIR VFQEDPNSDP QQPTFSLTAH LHQAHSQDVN CVAWNPKEPG LLASCSDDGE VAFWKYQRPE GL
Molecular Weight 19.9 kDa (177aa)
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_004795
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Johnstone RW, Wang J, et al. (1998). J Biol Chem. 273(18):10880-7.

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