| Product Name | CLTA, 1-218aa, Human |
|---|---|
| Description | Clathrin, light chain A, also known as CLTA, belongs to the clathrin family. Clathrin is a large, soluble protein composed of heavy and light chains. It is a major cytosolic coat protein in pits and vesicles originating from the plasma membrane and the trans-Golgi network. In receptor-mediated endocytosis, receptor proteins are captured by Clathrin-coated vesicles. CLTA is one of two clathrin light chain proteins which are believed to function as regulatory elements. Recombinant human CLTA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Clathrin, light chain A, LCA |
| Host | E.coli |
| Molecular Weight | 44.3kDa(399aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMAELDP FGAPAGAPGG PALGNGVAGA GEEDPAAAFL AQQESEIAGI ENDEAFAILD GGAPGPQPHG EPPGGPDAVD GVMNGEYYQE SNGPTDSYAA ISQVDRLQSE PESIRKWREE QMERLEALDA NSRKQEAEWK EKAIKELEEW YARQDEQLQK TKANNRAAEE AFVNDIDESS PGTEWERVAR LCDFNPKSSK QAKDVSRMRS VLISLKQAPL VH |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.15M NaCl |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Kirchhausen T., et al. (1987) Proc Natl Acad Sci USA. 84:8805-8809. Liu S H., et al. (1995) Cell. 83: 257-267. |
| Supplier | ARP |
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