Product Name | Cofilin 1, 1-166aa, Human, Recombinant |
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Description | Cofilin is a family of actin-binding proteins which disassembles actin filaments. It is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus. Recombinant human Cofilin, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques |
Synonyms | CFL1, CFL, Cofilin 1, CFL1, CFL, 18 kDa phosphoprotein(p18) 18 kDa phosphoprotein, CFL 1, Cofilin 1, Cofilin 1 non muscle, Cofilin non muscle isoform, P18. |
Host | E.coli |
Molecular Weight | 34.8 kDa (303 aa), confirmed by MALDI-TOF. |
Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCL SEDKKNIILE EGKEILVGDV GQTVDDPYAT FVKMLPDKDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS KDAIKKKLTG IKHELQANCY EEVKDRCTLA EKLGGSAVIS LEGKPL |
Tag | His-tag |
Reactivity | Human |
Applications | SDS-PAGE |
Form | Liquid, in 20 mM Tris-HCl buffer (pH 7.5) containing 5 mM DTT, 10% glycerol |
Concentration | 1 mg/ml (determined by Bradford assay) |
Purity | > 95% by SDS-PAGE |
Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
References |
Mannherz H.G., et al (2007). J. Mol. Biol. 366 (3)745-755. Olsen J.V., et al (2006). Cell. 127(3)635-648 Yang N., et al (1998) Nature. 393(6687)809-812 |
Supplier | ARP |
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