CRYGS, 1-178aa, Human

Proteins, Recombinant
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Product Name CRYGS, 1-178aa, Human
Background Crystallins are water soluble structural proteins found in the vertebrate eye. Mammalian crystallins are classified in three forms, designated alplha, beta and gamma. Crystallins, as the principal components of the lens, function to increase the refractive index of the eye during accommodation by forming high-molecular weight aggregates which maintain transparency. CRYGS, also known as beta-crystallin S, is exists as a monomer which does not aggregate. This gene encodes the most significant gamma-crystallin in adult eye lens tissue. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation. Recombinant human CRYGS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Beta-crystallin S, CRYG8
Species Human
Molecular Weight 26.5 kDa(230aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Concentration 1.0 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT
Purity > 95 % by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_060011
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Sun H., et al. (2005) J Med Genet. 42(9):706-10.
Jester JV., et al. (2008) Semin Cell Dev Biol. 19(2):82-93.

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