CRYM, 1-314aa, Human

Catalog
01-1832
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Proteins, Recombinant
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Product Name CRYM, 1-314aa, Human
Background Crystallin mu, also known as CRYM, is a member of the crystallin protein family. Crystallins are separated into two classes, taxon-specific and ubiquitous. This gene encodes a taxon-specific crystallin protein. The human CRYM gene maps to chromosome 16p13.11, and encodes a protein that is expressed in neural tissue, muscle, and kidney. Unlike other crystallins, CRYM does not perform a structural role in lens tissue, but rather it binds NADPH and thyroid hormone, which indicates that it may have other regulatory or developmental functions. Recombinant human CRYM, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms THBP, Crystallin mu CRYM, DFNA 40, DFNA40, Mu crystallin homolog, NADP regulated thyroid hormone binding protein, OTTHUMP00000115878.
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MSRVPAFLSA AEVEEHLRSS SLLIPPLETA LANFSSGPEG GVMQPVRTVV PVTKHRGYLG VMPAYSAAED ALTTKLVTFY EDRGITSVVP SHQATVLLFE PSNGTLLAVM DGNVITAKRT AAVSAIATKF LKPPSSEVLC ILGAGVQAYS HYEIFTEQFS FKEVRIWNRT KENAEKFADT VQGEVRVCSS VQEAVAGADV IITVTLATEP ILFGEWVKPG AHINAVGASR PDWRELDDEL MKEAVLYVDS QEAALKESGD VLLSGAEIFA ELGEVIKGVK PAHCEKTTVF KSLGMAVEDT VAAKLIYDSW SSGK
Molecular Weight 41.6 kDa (382aa)
Concentration 0.5mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 30% glycerol, 2mM EDTA, 0.1M NaCl
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_001879
Host E.coli
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Kim RY., et al. (1992). Proc Natl Acad Sci USA. 89(19):9292-6.
Muaders NW., et al. (1988) J Biol Chem. 263:15462-15466.

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