DFFA, 1-331aa, Human, Recombinant

Category: Proteins
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01-2144
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Product Name DFFA, 1-331aa, Human, Recombinant
Description DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA, also known as DNA fragmentation factor subunit alpha, is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Recombinant DFFA protein was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms FF-45, DFF1, ICAD, DNA fragmentation factor subunit alpha, A330085O09Rik, ICAD L, ICAD S, DFF 1, Caspase activated deoxyribonuclease inhibitor short form, DFF 45, DFF alpha, DFF35, DFF45, DFFA, Caspase-activated deoxyribonuclease inhibitor short form, Dffa DNA fragmentation factor, alpha subunit, DNA fragmentation factor 45 kDa subunit, DNA Fragmentation Factor Alpha Subunit, DNA fragmentation factor subunit alpha, DNA fragmentation factor, 45 kD, alpha subunit, DNA fragmentation factor, 45kDa, alpha polypeptide, DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1, RP23 121D17.3, DNA fragmentation factor, alpha subunit, DNAation factor 45 kDa subunit, H13, Inhibitor of CAD, Inhibitor of Caspase Activated DNase, MGC143066, OTTHUMP00000001903, OTTHUMP00000001904,
Host E.coli
Molecular Weight 30.3 kDa (264aa), confirmed by MALDI-TOF.
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MEVTGDAGVP ESGEIRTLKP CLLRRNYSRE QHGVAASCLE DLRSKACDIL AIDKSLTPVT LVLAEDGTIV DDDDYFLCLP SNTKFVALAS NEKWAYNNSD GGTAWISQES FDVDETDSGA GLKWKNVARQ LKEDLSSIIL LSEEDLQMLV DAPCSDLAQE LRQSCATVQR LQHTLQQVLD QREEVRQSKQ LLQLYLQALE KEGSLLSKQE ESKAAFGEEV DAVDTGISRE TSSDVALASH ILTALREKQA PELSLSSQDL ELVTKEDPKA LAVALNWDIK KTETVQEACE WELALRLQQT QSLHSLRSIS ASKASPPGDL QNPKRARQDP T
Reactivity Human
Applications SDS-PAGE
Form Liquid, in 20 mM Tris-HCl buffer (pH8.0) containing, 10% glycerol
Concentration 1 mg/ml (determined by Bradford assay)
Purity > 90% by SDS-PAGE
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
References McCarty JS., et al. (1999) Biochem Biophys Res Commun. 264(1):176-80.
Gu J., et al. (1999) J Biol Chem. 274(30):20759-62.
Supplier ARP

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