DFFA, 1-331aa, Human, Recombinant

Catalog
01-2144
$0.00
-
+
Proteins, Recombinant
Questions? Contact Us
More Information
Product Name DFFA, 1-331aa, Human, Recombinant
Background DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA, also known as DNA fragmentation factor subunit alpha, is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Recombinant DFFA protein was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms FF-45, DFF1, ICAD, DNA fragmentation factor subunit alpha, A330085O09Rik, ICAD L, ICAD S, DFF 1, Caspase activated deoxyribonuclease inhibitor short form, DFF 45, DFF alpha, DFF35, DFF45, DFFA, Caspase-activated deoxyribonuclease inhibitor short form, Dffa DNA fragmentation factor, alpha subunit, DNA fragmentation factor 45 kDa subunit, DNA Fragmentation Factor Alpha Subunit, DNA fragmentation factor subunit alpha, DNA fragmentation factor, 45 kD, alpha subunit, DNA fragmentation factor, 45kDa, alpha polypeptide, DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1, RP23 121D17.3, DNA fragmentation factor, alpha subunit, DNAation factor 45 kDa subunit, H13, Inhibitor of CAD, Inhibitor of Caspase Activated DNase, MGC143066, OTTHUMP00000001903, OTTHUMP00000001904,
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MEVTGDAGVP ESGEIRTLKP CLLRRNYSRE QHGVAASCLE DLRSKACDIL AIDKSLTPVT LVLAEDGTIV DDDDYFLCLP SNTKFVALAS NEKWAYNNSD GGTAWISQES FDVDETDSGA GLKWKNVARQ LKEDLSSIIL LSEEDLQMLV DAPCSDLAQE LRQSCATVQR LQHTLQQVLD QREEVRQSKQ LLQLYLQALE KEGSLLSKQE ESKAAFGEEV DAVDTGISRE TSSDVALASH ILTALREKQA PELSLSSQDL ELVTKEDPKA LAVALNWDIK KTETVQEACE WELALRLQQT QSLHSLRSIS ASKASPPGDL QNPKRARQDP T
Molecular Weight 30.3 kDa (264aa), confirmed by MALDI-TOF.
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 20 mM Tris-HCl buffer (pH8.0) containing, 10% glycerol
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # AAH07721
Host E.coli
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References McCarty JS., et al. (1999) Biochem Biophys Res Commun. 264(1):176-80.
Gu J., et al. (1999) J Biol Chem. 274(30):20759-62.

All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.

Hi, have questions?

An ARP Customer Service Representative is happy to help!

or Call (800) 832-2611