DHPS, 1-369aa, Human

Catalog
01-1797
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Proteins, Recombinant
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Product Name DHPS, 1-369aa, Human
Background DHPS, which belongs to the deoxyhypusine synthase family of proteins, is important for the first step in the hypusine biosynthesis pathway. This protein catalyzes the NAD-dependent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the EIF5A precursor protein to form the intermediate deoxyhypusine residue. Recombinant DHPS protein was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms MIG13, Deoxyhypusine synthase DHS, DS, Migration inducing gene 13.
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MEGSLEREAP AGALAAVLKH SSTLPPESTQ VRGYDFNRGV NYRALLEAFG TTGFQATNFG RAVQQVNAMI EKKLEPLSQD EDQHADLTQS RRPLTSCTIF LGYTSNLISS GIRETIRYLV QHNMVDVLVT TAGGVEEDLI KCLAPTYLGE FSLRGKELRE NGINRIGNLL VPNENYCKFE DWLMPILDQM VMEQNTEGVK WTPSKMIARL GKEINNPESV YYWAQKNHIP VFSPALTDGS LGDMIFFHSY KNPGLVLDIV EDLRLINTQA IFAKCTGMII LGGGVVKHHI ANANLMRNGA DYAVYINTAQ EFDGSDSGAR PDEAVSWGKI RVDAQPVKVY ADASLVFPLL VAETFAQKMD AFMHEKNED
Molecular Weight 47.5 kDa (440aa), confirmed by MALDI-TOF.
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 100mM NaCl, 10% glycerol
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_001921
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Umland TC, et al. (2004) J. Biol. Chem. 279 (27): 28697-705
Jones T, et al. (1996) Genomics. 35 (3): 635-7.

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