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|Product Name||Disulfide oxidoreductase (DsbA), Recombinant|
|Background||Dsb proteins (DsbA, DsbB, DsbC, and DsbD) catalyze formation and isomerization of protein disulfide bonds in the periplasm of Escherichia coli. DsbC is periplasmic enzyme known as a disulfide isomerase and can convert aberrant disulfide bonds to correct ones. DsbC consists of 236 amino acids containing signal peptide(1-20 amino acids). Recombinant DsbC was expressed in E. coli and purified by using conventional chromatography techniques.|
|Synonyms||dsbA, rpbB, Disulfide oxidoreductase (DsbA) E Coli, DsDNA-binding protein A, Double-stranded DNA-binding protein, Disulfide oxidoreductase A periplasmic protein disulfide isomerase I, Thiol disulfide interchange protein dsbA.|
|Amino Acid Sequence||AQYEDGKQYT TLEKPVAGAP QVLEFFSFFC PHCYQFEEVL HISDNVKKKL PEGVKMTKYH VNFMGGDLGK DLTQAWAVAM ALGVEDKVTV PLFEGVQKTQ TIRSASDIRD VFINAGIKGE EYDAAWNSFV VKSLVAQQEK AAADVQLRGV PAMFVNGKYQ LNPQGMDTSN MDVFVQQYAD TVKYLSEKK|
|Molecular Weight||8.5kDa (76aa), confirmed by MALDI-TOF.|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20 mM Tris-HCl buffer (pH 7.5) containing 50mM NaCl, 1mM DTT, 20% Glycerol|
|Purity||> 95% by SDS-PAGE|
|NCBI Accession #||NP_418297|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Charbonnier, J. B. et al. (1999). Protein Sci. 8:96-105
Inaba, K. et al. (2004). J. Biol. Chem. 279:6761-6768.
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