Product Name | ECI1, 42-302aa, Human |
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Description | ECI1 is a member of the hydratase/isomerase superfamily. The protein is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. Recombinant human ECI1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Synonyms | DCI |
Host | E.coli |
Molecular Weight |
10.7kDa (93aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher) |
Amino Acid Sequence |
MGSSHHHHHH SSGLVPRGSH MGSFGSQRVL VEPDAGAGVA VMKFKNPPVN SLSLEFLTEL VISLEKLEND KSFRGVILTS DRPGVFSAGL DLTEMCGRSP AHYAGYWKAV QELWLRLYQS NLVLVSAING ACPAGGCLVA LTCDYRILAD NPRYCIGLNE TQLGIIAPFW LKDTLENTIG HRAAERALQL GLLFPPAEAL QVGIVDQVVP EEQVQSTALS AIAQWMAIPD HARQLTKAMM RKATASRLVT QRDADVQNFV SFISKDSIQK SLQMYLERLK EEKG |
Tag | His-tag |
Reactivity | Human |
Applications | SDS-PAGE |
Form |
Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl |
Concentration | 1 mg/ml (determined by Bradford assay) |
Purity | > 90% by SDS-PAGE |
Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
References |
Janssen U., et al (1994). Genomics 23:223-228 Takahashi Y., et al (1994). Biochem. J. 304:849-852 |
Supplier | ARP |
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