ECI1, 42-302aa, Human

Category: Proteins
Catalog
01-P2445
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Product Name ECI1, 42-302aa, Human
Description ECI1 is a member of the hydratase/isomerase superfamily. The protein is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. Recombinant human ECI1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms DCI
Host E.coli
Molecular Weight 10.7kDa (93aa) confirmed by MALDI-TOF
(Molecular size on SDS-PAGE will appear higher)
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MGSFGSQRVL VEPDAGAGVA VMKFKNPPVN SLSLEFLTEL VISLEKLEND KSFRGVILTS DRPGVFSAGL
DLTEMCGRSP AHYAGYWKAV QELWLRLYQS NLVLVSAING ACPAGGCLVA LTCDYRILAD NPRYCIGLNE TQLGIIAPFW LKDTLENTIG
HRAAERALQL GLLFPPAEAL QVGIVDQVVP EEQVQSTALS AIAQWMAIPD HARQLTKAMM RKATASRLVT QRDADVQNFV SFISKDSIQK
SLQMYLERLK EEKG
Tag His-tag
Reactivity Human
Applications SDS-PAGE
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
0.1M NaCl
Concentration 1 mg/ml (determined by Bradford assay)
Purity > 90% by SDS-PAGE
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
References Janssen U., et al (1994). Genomics 23:223-228
Takahashi Y., et al (1994). Biochem. J. 304:849-852
Supplier ARP

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