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|Product Name||EDF1, 1-148aa, Human|
|Background||Endothelial differentiation-related factor 1, also known as EDF1 may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. Also, EDF1 binds calmodulin thorough its IQ domain and regulates nitric oxide synthase activity through calmodulin sequestration in the cytoplasm. Though ubiquitously expressed, EDF1 is most abundant in adult liver, heart, adipose tissues, intestine and pancreas. Recombinant human EDF1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Synonyms||EDF-1, MBF1, Endothelial differentiation-related factor 1|
|Amino Acid Sequence||MAESDWDTVT VLRKKGPTAA QAKSKQAILA AQRRGEDVET SKKWAAGQNK QHSITKNTAK LDRETEELHH DRVTLEVGKV IQQGRQSKGL TQKDLATKIN EKPQVIADYE SGRAIPNNQV LGKIERAIGL KLRGKDIGKP IEKGPRAKLE HHHHHH|
|Molecular Weight||33.5kDa (303aa) confirmed by MALDI-TOF|
|Concentration||1mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl|
|Purity||> 90% by SDS-PAGE|
|NCBI Accession #||NP_003783|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Mariotti M., et al. (2000) J Biol Chem. 275:24047-24051.
Dragoni I., et al. (1998) J Biol Chem. 273: 31119-31124.
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