EEF1A1, 1-462aa Human

Catalog
01-P2613
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Proteins, Recombinant
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Product Name EEF1A1, 1-462aa Human
Background EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. Recombinant human EEF1A1 protein is expressed in E.coli.
Synonyms Eukaryotic translation elongation factor 1 alpha 1, CCS-3, CCS3, EEF-1, EEF1A, eEF1A-1, EF-Tu, EF1A, FLJ25721, GRAF-1EF, HNGC:16303, PTI1
Species Human
Amino Acid Sequence MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG KGSFKYAWVL DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK NMITGTSQAD CAVLIVAAGV GEFEAGISKN GQTREHALLA YTLGVKQLIV GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK IGYNPDTVAF VPISGWNGDN MLEPSANMPW FKGWKVTRKD GNASGTTLLE ALDCILPPTR PTDKPLRLPL QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP GQISAGYAPV LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA IVDMVPGKPM CVESFSDYPP LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK ITKSAQKAQK AK
Molecular Weight 10.3 kDa (95aa)
Concentration 1mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_001393
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Meriin AB, Zaarur N, et al. (2012). J Cell Sci. 125(Pt 11):2665-74.
Itagaki K, Naito T, et al. (2012). J Biol Chem. 287(19):16037-46.

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