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|Product Name||EIF1, 1-113aa, Human|
|Background||EIF1, also known as eukaryotic translation initiation factor 1, is a universally conserved translation factor that is necessary for scanning and involved in initiation site selection. This protein promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Recombinant human EIF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Synonyms||A121, EIF-1, EIF1A, ISO1, SUI1, Eukaryotic translation initiation factor 1, EIF1,|
|Amino Acid Sequence||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMSA IQNLHSFDPF ADASKGDDLL PAGTEDYIHI RIQQRNGRKT LTTVQGIADD YDKKKLVKAF KKKFACNGTV IEHPEYGEVI QLQGDQRKNI CQFLVEIGLA KDDQLKVHGF|
|Molecular Weight||13.8 kDa (128aa), confirmed by MALDI-TOF.|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Form||Liquid, in Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol|
|Purity||> 90% by SDS-PAGE|
|NCBI Accession #||NP_005792|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Dennis MD., et al. (2009) J Biol Chem. 284(31):20615-28.
Pisarev AV., et al. (2007) Cell. 131(2):286-99.
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