| Product Name | EIF1AY, 1-144aa, Human |
|---|---|
| Description | EIF1AY is similar to eukaryotic translation initiation factor 1A (EIF1A). This protein seems to be required for maximal rate of protein biosynthesis. It enhances ribosome dissociation into subunits and is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. Recombinant human EIF1AY protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Synonyms | Eukaryotic translation initiation factor 1A, Y-linked, eIF-1A Y isoform, eukaryotic translation initiation factor 4C(eIF-4C) |
| Host | E.coli |
| Molecular Weight | 26.6 kDa (225aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMPKNKGK GGKNRRRGKN ENESEKRELV FKEDGQEYAQ VIKMLGNGRL EALCFDGVKR LCHIRGKLRK KVWINTSDII LVGLRDYQDN KADVILKYNA DEARSLKAYG ELPEHAKINE TDTFGPGDDD EIQFDDIGDD DEDIDDI |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol |
| Concentration | 0.25 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Mahbybyl Huq AH., et al. (2009) J Child Neurol. 24(10):1258-61 Page DC., et al (1997) Science. 278(5338):675-80. |
| Supplier | ARP |
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